Journal: BMC Cancer

Article Title: Role of Cancer Associated Fibroblast (CAF) derived miRNAs on head and neck malignancies microenvironment: a systematic review

doi: 10.1186/s12885-025-13965-9

Figure Lengend Snippet: Summary characteristics of included studies

Article Snippet: Qin, et al. [ , ] , 2019 , china , In vitro (human) and In vivo (animal) , head and neck cancer (HNC) , In vitro case sample: 1. 80 pairs of tumor and adjacent normal tissues were obtained from patients diagnosed with primary HNC and underwent initial surgery between September 2011 and June 2015; another 28 pairs were collected between July 2018 and September 2018 2. In another 40 HNC patients, plasma samples were collected one day before surgery and three days after tumor resection 3. SCC-4, SCC-9, SCC-25, CAL 27, and 293 T cells were purchased from the American Type Culture Collection (ATCC, USA), and the University of Maryland Dental School, USA kindly provided the human HNC cell lines HN4, HN6, and HN30 In vitro control sample: Plasma samples from 30 donors who had undergone physical examination at the Ninth People’s Hospital were selected as healthy controls In vivo case sample: A tumor-bearing model was constructed in BALB/C athymic nude mice (4 weeks old) , 1. Cell cultures 2. Immunofluorescence 3. Western blot analysis 4. MTT assay 5. CM preparation 6. Exosome isolation 7. Transmission electron microscopy 8. Fluorescent labeling and transfer of exosomes 9. Co-culture assay 10. Plasmid construction 11. Cell transfection 12. Biotin miRNA pull-down assay 13. RNA extraction and real-time PCR analysis 14. RIP assay 15. Luciferase analysis 16. Immunoprecipitation of miRNA targets 17. Tumorigenicity assay in vivo 18. Statistical analyses , • Cancer-associated fibroblasts (CAFs) are naturally resistant to cisplatin and play a crucial role in controlling the survival and growth of head and neck cancer cells by transferring active miR-196a from CAFs to tumor cells through exosomes • Exosomal miR-196a interacts with new targets, CDKN1B and ING5, to provide resistance to cisplatin in head and neck cancer cells. Eliminating exosome or exosomal miR-196a from cancer-associated fibroblasts (CAFs) restored head and neck cancer (HNC) sensitivity to cisplatin • Cancer-associated fibroblast-derived exosomes may employ heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) to assist in packaging miR-196a • Higher levels of plasma exosomal miR-196a are associated with reduced overall survival rates and resistance to therapy , The study discovered that miR-196a from CAF-derived exosomes causes resistance to cisplatin in head and neck cancer via affecting CDKN1B and ING5. This suggests that miR-196a might be a valuable indicator and a possible target for overcoming cisplatin resistance in head and neck cancer.

Techniques: In Vitro, Control, Formalin-fixed Paraffin-Embedded, Expressing, Quantitative RT-PCR, Cell Culture, Transfection, Western Blot, Migration, Biomarker Discovery, Isolation, Purification, Reverse Transcription, Polymerase Chain Reaction, Over Expression, Labeling, Transmission Assay, Electron Microscopy, RNA Extraction, Sequencing, Quantitative Proteomics, Real-time Polymerase Chain Reaction, Immunofluorescence, Immunohistochemistry, Luciferase, Gene Expression, Disruption, Reporter Assay, Immunodepletion, Amplification, Immunocytochemistry, Flow Cytometry, Derivative Assay, Immunohistochemical staining, Extraction, Microarray, Contraction Assay, Comparison, In Vivo, Ex Vivo, Staining, Invasion Assay, Tube Formation Assay, Fluorescence, In Situ Hybridization, Reverse Transcription Polymerase Chain Reaction, Inhibition, Apoptosis Assay, Enzyme-linked Immunosorbent Assay, Produced, Phospho-proteomics, Transformation Assay, Activation Assay, Cell Isolation, Transduction, Stable Transfection, DNA Methylation Assay, Cell Migration Assay, Plasmid Preparation, Zymography, Functional Assay, Translocation Assay, Clinical Proteomics, Construct, MTT Assay, Co-culture Assay, Pull Down Assay, Immunoprecipitation, Tumorigenicity Assay, Transferring, RNA Sequencing, Knockdown, Small Interfering RNA, Immunostaining, Protein-Protein interactions, Gradient Centrifugation, CCK-8 Assay, Injection, Protein Extraction